plasmid for the 8x supertopflash reporter Search Results


top flash  (Addgene inc)
96
Addgene inc top flash
Top Flash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/bio_rxiv__2025__02__03__636272-52-13-14?v=Addgene+inc
Average 96 stars, based on 1 article reviews
top flash - by Bioz Stars, 2026-07
96/100 stars
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paav cag egfp apobec1 yth  (Addgene inc)
93
Addgene inc paav cag egfp apobec1 yth
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Paav Cag Egfp Apobec1 Yth, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pmc11293556-148-49-60?v=Addgene+inc
Average 93 stars, based on 1 article reviews
paav cag egfp apobec1 yth - by Bioz Stars, 2026-07
93/100 stars
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addgene 16495 pstf  (Addgene inc)
93
Addgene inc addgene 16495 pstf
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Addgene 16495 Pstf, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pm36346705__jm2c01199_si_001-86-37-44?v=Addgene+inc
Average 93 stars, based on 1 article reviews
addgene 16495 pstf - by Bioz Stars, 2026-07
93/100 stars
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mutant reporter m51 super 89 fopflash  (Addgene inc)
94
Addgene inc mutant reporter m51 super 89 fopflash
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Mutant Reporter M51 Super 89 Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pm27350215-76-20-30?v=Addgene+inc
Average 94 stars, based on 1 article reviews
mutant reporter m51 super 89 fopflash - by Bioz Stars, 2026-07
94/100 stars
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8x gtiic tead luciferase reporter plasmid  (Addgene inc)
96
Addgene inc 8x gtiic tead luciferase reporter plasmid
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
8x Gtiic Tead Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pmc09926304-283-1-11?v=Addgene+inc
Average 96 stars, based on 1 article reviews
8x gtiic tead luciferase reporter plasmid - by Bioz Stars, 2026-07
96/100 stars
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tgp endogenous canonical wnt reporter  (Addgene inc)
93
Addgene inc tgp endogenous canonical wnt reporter
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Tgp Endogenous Canonical Wnt Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pmc07455709-240-9-22?v=Addgene+inc
Average 93 stars, based on 1 article reviews
tgp endogenous canonical wnt reporter - by Bioz Stars, 2026-07
93/100 stars
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pabbeetm-fh plasmid  (GenScript corporation)
90
GenScript corporation pabbeetm-fh plasmid
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Pabbeetm Fh Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pmc09328775-215-18-26?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
pabbeetm-fh plasmid - by Bioz Stars, 2026-07
90/100 stars
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pmt123 ha 8x ubiquitin  (Addgene inc)
96
Addgene inc pmt123 ha 8x ubiquitin
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Pmt123 Ha 8x Ubiquitin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pmc07567074-247-12-16?v=Addgene+inc
Average 96 stars, based on 1 article reviews
pmt123 ha 8x ubiquitin - by Bioz Stars, 2026-07
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luciferase reporter plasmid 8x-cbf1-luc  (Promega)
90
Promega luciferase reporter plasmid 8x-cbf1-luc
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Luciferase Reporter Plasmid 8x Cbf1 Luc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/10__1128_slash_mcb__20__11__3928___3941__2000-129-1-23?v=Promega
Average 90 stars, based on 1 article reviews
luciferase reporter plasmid 8x-cbf1-luc - by Bioz Stars, 2026-07
90/100 stars
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wnt reporterm50super 8x topflash  (Addgene inc)
90
Addgene inc wnt reporterm50super 8x topflash
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Wnt Reporterm50super 8x Topflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/10__1158_slash_1535___7163__mct___18___0873-107-76-112?v=Addgene+inc
Average 90 stars, based on 1 article reviews
wnt reporterm50super 8x topflash - by Bioz Stars, 2026-07
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curt davey  (Addgene inc)
93
Addgene inc curt davey
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
Curt Davey, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/bio_rxiv__2025__07__31__667981-43-12-14?v=Addgene+inc
Average 93 stars, based on 1 article reviews
curt davey - by Bioz Stars, 2026-07
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m50 super 8x topflash reporter plasmid  (Promega)
90
Promega m50 super 8x topflash reporter plasmid
Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to <t>APOBEC1,</t> the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.
M50 Super 8x Topflash Reporter Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+for+the+8x+supertopflash+reporter/pmc06945285-108-12-27?v=Promega
Average 90 stars, based on 1 article reviews
m50 super 8x topflash reporter plasmid - by Bioz Stars, 2026-07
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Image Search Results


Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to APOBEC1, the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.

Journal: Genome Research

Article Title: Single-cell discovery of m 6 A RNA modifications in the hippocampus

doi: 10.1101/gr.278424.123

Figure Lengend Snippet: Improved m 6 A bulk RNA-seq detection in HEK293T cells. ( A ) Schematic diagram of m 6 A detection with bulk RNA-seq in cultured cells. The YTH protein domain binds to m 6 A. When bound to APOBEC1, the APOBEC1 protein converts C-to-U in the vicinity of m 6 A. This results in a C-to-T mutation in cDNA. C-to-T mutations detected by RNA sequencing are indicative of m 6 A RNA modifications. ( B , left ) Immunofluorescence (IF) of HEK293T cells transfected with Apobec1-Yth-HA-Egfp ( Yth-HA-E ) or Egfp-Apobec1-Yth-HA (E-YTH-HA ). Scale bar, 20 μm. Representative images are shown. ( Right ) Quantification of EGFP and HA overlap. (PCC) Pearson correlation coefficient. ( C ) Number of C-to-T editing events identified in each bulk RNA-seq HEK293T cell replicate for Yth-E and E-Yth plasmids. Editing events identified in at least two replicates were considered for downstream analyses. The data were obtained following Apobec1-Yth-Egfp or Egfp-Apobec1-Yth transfection and EGFP FACS sorting. n = 3. (Rep) separately cultured replicate. ( D ) Metagene analysis showing m 6 A site counts along transcripts for Yth-E and E-Yth bulk RNA-seq results. Nine percent of all m 6 A sites occur in the first 10% of the 3′ UTR following the TTS for YTH-E and 11% for E-YTH, respectively. Shown percentage indicates number of m 6 A sites upstream of, within, and downstream from coding sequence (CDS). ( E ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) for YTH-E and E-YTH.

Article Snippet: An Adeno-associated plasmid pAAV- Cag-Egfp-Wpre-Sv40 (gift from Minmin Luo) was used as the backbone to generate the viral expression constructs pAAV- Cag-Apobec1-Yth-Egfp ( Yth-E , Addgene 209322), pAAV- Cag - Apobec1 - Yth mut - Egfp ( Yth mut -E , Addgene 209323), pAAV- Cag-Apobec1 - Egfp (Addgene 209324), pAAV- Cag - Egfp - Apobec1 - Yth ( E-Yth , Addgene 209319), pAAV- Cag-Egfp-Apobec1 - Yth mut ( E - Yth mut , Addgene 209320), and pAAV -Cag-Egfp-Apobec1 (Addgene 209321).

Techniques: RNA Sequencing, Cell Culture, Mutagenesis, Immunofluorescence, Transfection, Sequencing

Detection of m 6 A with bulk RNA-seq in mouse hippocampus. ( A ) Schematic diagram of bulk RNA-seq in the mouse brain. The Egfp-Apobec1-Yth is packaged into AAV viruses to infect brain cells. EGFP-positive cells are isolated from the hippocampus and processed for C-to-U edit and m 6 A site identification. ( B ) Confocal image of mouse hippocampus after AAV infection. Representative image of E-YTH is shown. Half-brain image: Scale bar, 1 mm. Hippocampus image: Scale bar, 400 μm. ( C ) Number of overlapping C-to-U editing events identified by RNA-seq in hippocampus following Egfp-Apobec1-Yth and Egfp-Apobec1-Yth mut AAV virus injection and EGFP FACS sorting. Editing events identified in at least two replicates were considered for downstream analyses. n = 3, (Rep) Biological replicates from different animals. ( D ) Pie chart showing m 6 A localization identified by E-Yth in mouse hippocampus. (TTS) Transcription termination site. ( E ) Metagene analysis showing C-to-U edit scaled density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) in E-YTH, E-YTH mut , E-APOBEC1, and wild-type (WT) samples. The peak value for E-YTH is 2716 editing events. ( F ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt). m 6 A sites were obtain after eliminating background from E-YTH editing sites. m 6 A peak density occurs 118 nt downstream from the stop codon. ( G ) Histogram of m 6 A site counts over mutation per read (m/k) ratio. Minimum threshold: 5%.

Journal: Genome Research

Article Title: Single-cell discovery of m 6 A RNA modifications in the hippocampus

doi: 10.1101/gr.278424.123

Figure Lengend Snippet: Detection of m 6 A with bulk RNA-seq in mouse hippocampus. ( A ) Schematic diagram of bulk RNA-seq in the mouse brain. The Egfp-Apobec1-Yth is packaged into AAV viruses to infect brain cells. EGFP-positive cells are isolated from the hippocampus and processed for C-to-U edit and m 6 A site identification. ( B ) Confocal image of mouse hippocampus after AAV infection. Representative image of E-YTH is shown. Half-brain image: Scale bar, 1 mm. Hippocampus image: Scale bar, 400 μm. ( C ) Number of overlapping C-to-U editing events identified by RNA-seq in hippocampus following Egfp-Apobec1-Yth and Egfp-Apobec1-Yth mut AAV virus injection and EGFP FACS sorting. Editing events identified in at least two replicates were considered for downstream analyses. n = 3, (Rep) Biological replicates from different animals. ( D ) Pie chart showing m 6 A localization identified by E-Yth in mouse hippocampus. (TTS) Transcription termination site. ( E ) Metagene analysis showing C-to-U edit scaled density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt) in E-YTH, E-YTH mut , E-APOBEC1, and wild-type (WT) samples. The peak value for E-YTH is 2716 editing events. ( F ) Metagene analysis showing m 6 A density 500 nt 5′ and 500 nt 3′ from stop codon (0 nt). m 6 A sites were obtain after eliminating background from E-YTH editing sites. m 6 A peak density occurs 118 nt downstream from the stop codon. ( G ) Histogram of m 6 A site counts over mutation per read (m/k) ratio. Minimum threshold: 5%.

Article Snippet: An Adeno-associated plasmid pAAV- Cag-Egfp-Wpre-Sv40 (gift from Minmin Luo) was used as the backbone to generate the viral expression constructs pAAV- Cag-Apobec1-Yth-Egfp ( Yth-E , Addgene 209322), pAAV- Cag - Apobec1 - Yth mut - Egfp ( Yth mut -E , Addgene 209323), pAAV- Cag-Apobec1 - Egfp (Addgene 209324), pAAV- Cag - Egfp - Apobec1 - Yth ( E-Yth , Addgene 209319), pAAV- Cag-Egfp-Apobec1 - Yth mut ( E - Yth mut , Addgene 209320), and pAAV -Cag-Egfp-Apobec1 (Addgene 209321).

Techniques: RNA Sequencing, Isolation, Infection, Virus, Injection, Mutagenesis